2 edition of Kinetic studies of rabbit muscle phosphofructokinase found in the catalog.
Kinetic studies of rabbit muscle phosphofructokinase
Anita Sia Kee
Written in English
|Statement||by Anita Sia Kee|
|The Physical Object|
|Pagination||59 leaves :|
|Number of Pages||59|
Utilization of carbohydrate and fatty acids is strongly influenced by rates of conÂ sumption and production of high energy compounds in cardiac muscle. In wellÂ oxygenated hearts, fatty acid has been identified as the preferred substrate by both in vivo and in vitro studies (for review see ). Availability of fatty acid suppresses glucose utilization by inhibition of several steps in. Integrating Proteomics and Enzyme Kinetics Reveals Tissue-Specific Types of the Glycolytic and Gluconeogenic PathwaysCited by:
Protein structure of PKM1 and PKM2. Mammalian pyruvate kinase is a tetrameric protein of identical subunits, which are arranged in a dimer-of-dimers configuration (Figure 2).Each monomer contains one active site and is composed of three main domains – designated A, B, and C – plus a small N-terminal domain [24, 25].The A domain is the largest domain and is a symmetric α 8 /β 8 TIM Cited by: Interestingly, in CCDS patients, creatine deficiency is much less severe in muscle than in brain. Although creatine levels in skeletal muscle have not been investigated extensively in CCDS patients and insights are based on case studies only, creatine content of skeletal muscle was measured in vivo by proton MRS and in vitro in a muscle biopsy /5.
Studies of an Isolated Mammalian Heart Preparation Capable of Performing Work for Prolonged Periods: T no. Biology: D'Angelo, Louis: The Binding of Cyclic Nucleotides by Allosteric Rabbit Muscle Phosphofructokinase Using a Modified Assay Technique: no. . ATP and Brain Function. Maria Erecińska and Ian A. Silver. Journal of Cerebral Blood Flow & Metabolism 9: 1, perikarya and synaptosomes from rabbit brain cortex. Danforth, WH, () Effect of pH on the kinetics of frog muscle by:
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This chapter discusses a procedure for isolating phosphofructokinase from rabbit muscle. In the procedure, the extract of rabbit muscle was brought to a 10% concentration of isopropanol at 40°C.
The muscle from one rabbit gave about mg of the purified enzyme in a yield of 60%. Structural Properties A. Rabbit Muscle Phosphofructokinase Catalytically active rabbit muscle phosphofructokinase has a mini mum molecular weight of( S) but can be dis sociated into a protein with a molecular weight ofwith a PHOSPHOFRUCTOKINASE: STRUCTURE AND FUNCTION sedimentation constant of 7 S by decreasing Cited by: Schiltz, Haiqing Fu, "Transcriptional Studies of the Muscle-Specific Expression of the Rabbit Muscle Phosphofructokinase Gene." ().
LSU Historical Dissertations and Theses. Author: Haiqing Fu Schiltz. Aaronson, R.P., Frieden, C.: Rabbit muscle phosphofructokinase: Studies on the behaviour of the enzyme at pH 8, pH 6, and intermediate pH values. J Cited by: Site-directed mutagenesis of rabbit muscle phosphofructokinase cDNA: Mutations at glutamine affect the allosteric properties of the enzyme.
Previous studies showed that rabbit muscle phosphofructokinase-1 (PFK-1) activity losses due to dilution, due to inhibition by ascorbate, and due to some lithium salts were prevented by rabbit. Brooks, S.P.J.; Storey, K.B.: Phosphofructokinase from a vertebrate facultative anaerobe: effects of temperature and anoxia on the kinetic parameters of the purified enzyme from.
Triose phosphate isomerase is a dimer of identical subunits, each of which is made up of about amino acid residues. The three-dimensional structure of a subunit contains eight α-helices on the outside and eight parallel β-strands on the inside. In the illustration, the ribbon backbone of each subunit is colored in blue to red from N-terminus to : BRENDA entry.
If the address matches an existing account you will receive an email with instructions to reset your password. Control of PPi and ATP-phosphofructokinase in sucrose metabolism in mature grapefruit.
Van Praag E, Zehavi U, Goren R. Abstracts of Papers of the American Chemical Society () vol. 7-carb (1) Use of 3-D computer modelling and kinetic studies to analyze grapefruit pyrophosphate-dependent phosphofructokinase. Morpheeins are proteins that can form two or more different homo-oligomers (morpheein forms), but must come apart and change shape to convert between forms.
The alternate shape may reassemble to a different oligomer. The shape of the subunit dictates which oligomer is formed. Each oligomer has a finite number of subunits (stoichiometry).Morpheeins can interconvert between forms under.
Amino Acid Composition and Subunit Structure of Rabbit Muscle Aldolase, Biochim Biophys Acta, Sia, C., and Horecker, B.: The Molecular Weight of Rabbit Muscle Aldolase and the Properties of the Subunits in Acid Solution, Arch Biochem Biophys, This reference volume follows the Fructose-2, 6-P2 story from its discovery in to current studies on the enzyme systems responsible for its synthesis and degradation.
The book begins with a historical perspective on the discovery of the compound and then proceeds to its chemistry and number of derivatives. It includes a detailed treatment of the role of the compound in the regulation of.
laboratory have carried out studies on phosphofructokinase (E.C. ) from rabbit muscle, which is one of the key regulatory enzymes of the glycolytic pathway1*•4. Through the use of structurally locked analogues of the tx- and p-forms of its carbohydrate substrate, it was established that the enzyme acts on the : Ronald Joseph Voll.
Molecular and cellular biology methodology is traditionally based on the reasoning called “the mechanistic explanation”.
In practice, this means identifying and selecting correlations between biological processes which result from our manipulation of a biological system.
In theory, a successful application of this approach requires precise knowledge about all parameters of a studied by: 3. Thermodynamic linkages in rabbit muscle pyruvate kinase: kinetic, equilibrium, and structural studies Robert W.
Oberfelder, Lucy L. Lee, and James C. Lee Biochemistry 23 (17), A vacuolar acid phosphatase (APase) that accumulates during phosphate (Pi) starvation of Arabidopsis (Arabidopsis thaliana) suspension cells was purified to homogeneity.
The final preparation is a purple APase (PAP), as it exhibited a pink color in solution (A max = nm). It exists as a kD homodimer composed of kD glycosylated subunits that cross-reacted with an anti.
A severe, progressive myopathy developed in an year-old, phosphofructokinase (PFK)-deficient, male, English Springer Spaniel dog. Results from a routine neurological examination were normal.
Examination of histologic sections of skeletal muscle Cited by: The activation of rabbit skeletal muscle phosphorylase kinase requires the binding of 3 Ca2+ per δ subunit Danielle Burger, Jos A.
Cox, Edmond H. Fischer, Eric A. Stein Biochemical and Biophysical Research Communications (2), The book also offers the reader important knowledge on topics like embryonic development, muscle organization, energy metabolism, structure of the muscle fiber, and mechanisms of fatigue.
Outstanding number of photographs, illustrations and graphs assist the reader in understanding with great visual aids. This review provides an overview of the current knowledge of eukaryotic 6-phosphofructokinase based on recent crystal structures, kinetic analyses and site-directed mutagenesis data with special focus on the molecular architecture and the structural basis of allosteric by: Kinetic mechanisms have played a major role in defining the metabolic pathways, the mechanistic action of enzymes, and even the processing of genetic material.
The Handbook of Biochemical Kinetics provides the "underlying scaffolding" of logic for kinetic approaches to distinguish rival models or mechanisms. The handbook also comments on.Metabolism sustains life through enzyme-catalyzed chemical reactions within the cells of all organisms.
The coupling of catalytic function to the structural organization of enzymes contributes to the kinetic optimization important to tissue-specific and whole-body function. This coupling is of paramount importance in the role that muscle plays in the success of by: